Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Monoclonal Antibody (KBA.62).

Formalin-fixed, paraffin-embedded human Tongue stained with Melanoma Monoclonal Antibody (KBA.62).

SDS-PAGE Analysis Purified Melanoma Monoclonal Antibody (KBA.62). Confirmation of Purity and Integrity of Antibody.

KBA.62 (Melanoma Associated Antigen) [KBA.62]

Name: KBA.62 (Melanoma Associated Antigen)
SKU: MSM1-895
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node.

Printable

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

N/A

Gene Name

N/A

Mol. Weight of Antigen

135 and 128kDa and two weak bands of 88 and 73kDa), Multiple (140

Immunogen

Human KAL cells derived from lymph node metastasis of malignant melanoma

Database Links

Entrez Gene ID

Not Known

Unigene

Not Known

SwissProt

N/A

Additional Information

Clone

KBA.62

Human Gene Symbol

Not Known

Chromosome Location

Not Known

Known Applications & Suggested Dilutions

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

E Cohen-Knafo, T al Saati, J Aziza, E Ralfkiaer, J Selves, B Gorguet, and G Delsol. Production and characterisation of an antimelanoma monoclonal antibody KBA.62 using a new melanoma cell line reactive on paraffin wax embedded sections. J Clin Pathol. 1995; 48(9): 826-831.
Cecile Pages et. al. KBA.62: a useful marker for primary and metastatic melanomas. Human Pathology 39(8):1136-1142; 2008.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.