Western blotting is a fundamental technique in molecular biology and protein research, serving as a powerful tool for detecting and quantifying specific proteins within a complex mixture [see link for Protocol]. Specificity testing, involving Western blotting, helps verify that an antibody binds to a specific antigen while minimizing non-specific binding, preventing false-positive results that could lead to erroneous conclusions in the realms of basic, diagnostic, and clinical research. Further validation of antibody specificity can be achieved by replicating the Western blot experiment using different antibody lots or techniques to validate the results further and/or including controls such as knockdown or knockout cell lines or tissues as additional validation steps.
Antibody validation for western blot applications requires a comprehensive approach. At NeoBiotechnologies, we assess factors such as specificity, sensitivity, and reproducibility, implement best practices, follow established guidelines, and adhere to validation standards to guarantee that our antibodies are reliable tools that enhance the accuracy of Western blot experiments.
Neobiotechnologies’ dedication to antibody validation underscores the importance of precision and reliability in scientific endeavors. Explore our comprehensive range of validated antibodies tailored for western blot applications, and embark on a journey of discovery with confidence. Your research deserves the clarity and accuracy that Neobiotechnologies’ validated antibodies bring to the forefront of western blot applications.