Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Monoclonal Antibody (KRTH/1076).

Formalin-fixed, paraffin-embedded Rat Stomach stained with Cytokeratin, HMW Monoclonal Antibody (KRTH/1076).

Formalin-fixed, paraffin-embedded Rat Oviduct with Cytokeratin, HMW Monoclonal Antibody (KRTH/1076).

SDS-PAGE Analysis Purified Cytokeratin, HMW MAb (KRTH/1076). Confirmation of Purity and Integrity of Antibody.

Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) [KRTH/1076]

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Summary

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

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Product Properties & Targets

Host
Mouse
Species Reactivity
Human, Rat
Research Areas
Autophagy
Isotype
IgG1
Cellular Localization
Cytosol, Extracellular exosome, intermediate filament, keratin filament, Nucleus
Gene Name
KRT76
Mol. Weight of Antigen
67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8)
Immunogen
Recombinant human KRT76 protein

Database Links

Entrez Gene ID
51350
Unigene
654392
SwissProt
Q01546

Additional Information

Clone
KRTH/1076
Human Gene Symbol
KRT76
Chromosome Location
12q13.13

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  • Tseng SCG et. al. Cell 1982; 30361.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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