Cytokeratin, Type II (Epithelial Marker) [rKRTH/6617]

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Summary

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Product Properties & Targets

Host
Species Reactivity
Isotype
Gene Name
KRT76
Mol. Weight of Antigen
66/65/64/57/62/60/51/54kDa
Immunogen
Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
rKRTH/6617
Human Gene Symbol
KRT76
Chromosome Location
12q13.13

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

  • Spagnolo DV, et al. Am J Clin Pathol. 1985 Dec;84(6):697-704.
  • Eichner R, et al. J Cell Biol. 1984 Apr;98(4):1388-96.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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