Formalin-fixed, paraffin-embedded human sebaceous gland stained with CK Type II Recombinant Mouse Monoclonal Antibody (rKRTH/6617).

Formalin-fixed, paraffin-embedded human skin stained with CK Type II Recombinant Mouse Monoclonal Antibody (rKRTH/6617).

SDS-PAGE Analysis Purified CK Type 2 Recombinant Mouse Monoclonal (rKRTH/6617). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

Cytokeratin, Type II (Epithelial Marker) [rKRTH/6617]

Name: Cytokeratin, Type II (Epithelial Marker)
SKU: MSM45-6617
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies Recombinant Mouse Monoclonals Recombinants

Summary

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Gene Name

KRT76

Mol. Weight of Antigen

66/65/64/57/62/60/51/54kDa

Immunogen

Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins

Database Links

Entrez Gene ID

51350

Unigene

654392

SwissProt

Q01546

Additional Information

Clone

rKRTH/6617

Human Gene Symbol

KRT76

Chromosome Location

12q13.13

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

Spagnolo DV, et al. Am J Clin Pathol. 1985 Dec;84(6):697-704.
Eichner R, et al. J Cell Biol. 1984 Apr;98(4):1388-96.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.