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Cytokeratin, Basic (Type II or HMW) Antibody [34BE12]

In Stock
Best Seller
Catalog Number Formulation Size Price
MSM3-446-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM3-446-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM3-446-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Gene Name
Positive Control
HeLa, MCF-7, PC12 cells, Prostate carcinoma., Skin
Immunogen
Solubilized keratin extract from human stratum corneum
Alternate Names
Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
34BE12
Chromosome Location
12q13.13
Mol. Weight of Antigen
50-67kDa

Functions

  • Probably contributes to terminal cornification.

Key References

  • Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58
  • Varma M et. al. Mod Pathol 1999;12(5):472-8

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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