Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin, HMW Mouse Monoclonal Antibody (DE-SQ).

Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin, HMW Mouse Monoclonal Antibody (DE-SQ).

Printable Datasheet

Cytokeratin, Basic (Type II or HMW) [DE-SQ]

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Summary

This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

Product Properties & Targets

Host
Mouse
Species Reactivity
Human
Isotype
IgG1
Gene Name
KRT76
Mol. Weight of Antigen
50-67kDa
Immunogen
Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction

Database Links

Entrez Gene ID
51350
Unigene
654392
SwissProt
Q01546

Additional Information

Clone
DE-SQ
Human Gene Symbol
KRT76
Chromosome Location
12q13.13

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58
  • Varma M et. al. Mod Pathol 1999;12(5):472-8

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Related products

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

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