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Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker) Antibody [CCND1/7868]

In Stock
Catalog Number Formulation Size Price
595-MSM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
595-MSM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
595-MSM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human renal cell carcinoma stained Cyclin D1 Mouse Monoclonal Antibody (CCND1/7868). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained Cyclin D1 Mouse Monoclonal Antibody (CCND1/7868). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human spleen stained Cyclin D1 Mouse Monoclonal Antibody (CCND1/7868). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human spleen stained Cyclin D1 Mouse Monoclonal Antibody (CCND1/7868). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, Immunology, AKT Signaling, Bladder Cancer, Colon Cancer, Cytokine Signaling, Infectious Disease, Lung Cancer, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Nucleus.
Gene Name
CCND1
Positive Control
breast or bladder carcinomas.|Human mantle cell lymphoma
Immunogen
Recombinant fragment (around aa200-295) of Cyclin D1 protein (exact sequence is proprietary)
Alternate Names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, B cell CLL/lymphoma 1; B cell leukemia 1; B-cell lymphoma 1 protein; BCL-1 oncogene; CCND1 protein; CCND1/FSTL3 fusion gene; CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene; CCND1/PTH fusion gene; G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene

Database Links

Entrez Gene ID
595
SwissProt
P24385Human

Additional Information

Clone
CCND1/7868
Chromosome Location
11q13.2
Mol. Weight of Antigen
36kDa

Related Antibodies

View All CCND1 Antibodies

Functions

  • Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162, PubMed:33854235). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Hypophosphorylates RB1 in early G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:1833066, PubMed:1827756, PubMed:8302605, PubMed:19412162). Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed:15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed:16569215, PubMed:18417529).

Key References

  • Baldin V, et al. Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes and Development, 1993, 7(5):812-21.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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