Printable Datasheet

Cyclin D1 (G1-Cyclin & Mantle Cell Marker) [DCS-6]

Clear

Summary

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Product Properties & Targets

Host
Mouse
Species Reactivity
Human, Monkey, Mouse, Rat
Research Areas
Cancer, Cardiovascular
Isotype
IgG2a, kappa
Cellular Localization
nuclear
Gene Name
CCND1
Positive Control
BT474|HT29|Jurkat or A431 cells. ~60% of mantle cell lymphomas and ~40% of breast carcinomas are positive.|MCF-7|MDA-MB-453|Ramos|SKBR3|T47D|ZR75
Immunogen
Human recombinant full length cyclin D1 protein
Alternate Names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene

Database Links

Entrez Gene ID
595
Unigene
523852; 667996
SwissProt
P24385

Additional Information

Clone
DCS-6
Human Gene Symbol
CCND1
Chromosome Location
11q13.2
Mol. Weight of Antigen
36kDa

Functions

  • Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162, PubMed:33854235). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Hypophosphorylates RB1 in early G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:1833066, PubMed:1827756, PubMed:8302605, PubMed:19412162). Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed:15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed:16569215, PubMed:18417529).

Known Applications & Suggested Dilutions

  • Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100 for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Key References

  • Lukas J, et. al. Oncogene, 1994, 9(3):707-18.
  • Gillett C, et. al. Cancer Research, 1994, 54(7):1812-7.
  • Bartkova J, et. al. Journal of Pathology, 1994, 172(3):237-45.

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Cyclin D1 (G1-Cyclin & Mantle Cell Marker)”

Your email address will not be published. Required fields are marked *

Related Antibodies

View All CCND1 Antibodies

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies

Company Info
Antibodies
View All
Hosts
View All
Isotype
View All
Resources
Newsletter Signup NeoBiotechnologies
Updates & Sales

$0.00
510-376-5603