Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained Cyclin D1 Recombinant Rabbit Monoclonal Antibody (CCND1/3370R).

Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained Cyclin D1 Recombinant Rabbit Monoclonal Antibody (CCND1/3370R).

SDS-PAGE Analysis Purified Cyclin D1 Recombinant Rabbit Monoclonal Antibody (CCND1/3370R). Confirmation of Purity and Integrity of Antibody.

Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker) [CCND1/3370R]

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Summary

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

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Product Properties & Targets

Host
Rabbit
Species Reactivity
Human
Isotype
IgG
Cellular Localization
Cytoplasm, Nucleus, Nucleus membrane
Gene Name
CCND1
Mol. Weight of Antigen
36kDa
Immunogen
Synthetic peptide corresponding to residues within aa 270-295 of Cyclin D1

Database Links

Entrez Gene ID
595
Unigene
523852 & 667996
SwissProt
P24385

Additional Information

Clone
CCND1/3370R
Human Gene Symbol
CCND1
Chromosome Location
11q13.2

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Baldin V, et al. Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes and Development, 1993, 7(5):812-21.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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