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Cyclin D1 (G1-Cyclin & Mantle Cell Marker) Antibody [CCND1/809]

Additional information

Catalog Number: 595-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
595-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
595-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
595-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cyclin D1 Antibody in Immunohistochemistry (IHC (P))

Cyclin D1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

Cyclin D1 Antibody in Immunohistochemistry (IHC (P))

Cyclin D1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

Cyclin D1 Antibody in Flow Cytometry (FC)

Cyclin D1 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of human Cyclin D1 on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

Cyclin D1 Antibody in Flow Cytometry (FC)

Cyclin D1 Antibody in Flow Cytometry (FC)

Flow cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled Cyclin D1 Mouse Monoclonal Antibody (CCND1/809).

Cyclin D1 Antibody in SDS-PAGE

Cyclin D1 Antibody in SDS-PAGE

Purified Cyclin D1 Mouse Monoclonal Antibody (CCND1/809). Confirmation of Integrity and Purity of Antibody

CCND1 Antibody in SDS-PAGE

CCND1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified G1/S-specific cyclin-D1 Mouse Monoclonal Antibody (CCND1/809). Confirmation of Purity and Integrity of Antibody.

CCND1 Antibody in SDS-PAGE

CCND1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified G1/S-specific cyclin-D1 Mouse Monoclonal Antibody (CCND1/2593). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Clone
CCND1/809
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus, Nucleus membrane
Gene Name
CCND1
Positive Control
T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. ~60% of mantle cell lymphomas and ~40% of breast carcinomas are positive.
Immunogen
Recombinant human CCND1 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162, PubMed:33854235). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Hypophosphorylates RB1 in early G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:1833066, PubMed:1827756, PubMed:8302605, PubMed:19412162). Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed:15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed:16569215, PubMed:18417529).
Key References
  • Baldin V; Lukas J; Marcote MJ; Pagano M; Draetta G. Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes and Development, 1993, 7(5):812-21.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
595
SwissProt
P24385
Chromosome Location
11q13.2
Mol. Weight of Antigen
36kDa
Alternate Names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene

Summary

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

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