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CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody [ICO-115]

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Flow cytometry of KG-1 cells using CD34 Monoclonal Antibody (ICO-115) (red) and isotype control Ab (green).

Flow cytometry of KG-1 cells using CD34 Monoclonal Antibody (ICO-115) (red) and isotype control Ab (green).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human, Rat
Research Areas
Hypoxia, Immunology, Endothelial Cell Marker, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell surface
Gene Name
CD34
Positive Control
KG-1 cells. Human tonsil or angiosarcoma.
Immunogen
Blast cells of a chronic myeloid leukemia patient
Alternate Names
Hematopoietic progenitor cell antigen CD34, Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Database Links

Entrez Gene ID
947
SwissProt
P28906

Additional Information

Clone
ICO-115
Chromosome Location
1q32.2
Mol. Weight of Antigen
90-110kDa

Related Antibodies

View All CD34 Antibodies

Functions

  • Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Key References

  • Frolova, E., Baryshnikov A.Y., Frenkel M. et al., Monoclonal antibody ICO-115 to human CD34 antigen. Herald of the Cancer Research Center of RAMS. Suppl, p. 10-12, 1994.
  • Sato T et al. Blood 94:2548-2554 (1999).
  • Felshow DM et al. Blood 97:3768-3775 (2001).
  • Baryshnikov A, and Tonevitsky A, Monoclonal antibodies in laboratory and clinic. Thesis p212, 1997.
  • Kishimoto et al., Leucocyte typing Gariand Publishing. 1997.

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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