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Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody [HPCA1/4393R]

In Stock
Catalog Number Formulation Size Price
947-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
947-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
947-RBM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD34 (also named myeloid progenitor cell antigen) is a heavily glycosylated type I transmembrane protein. There are two forms of the CD34 protein, resulting from alternative splicing. The functions of CD34 is largely unknown, but recent evidence suggests a role for CD34 in cell adhesion and inhibition of hematopoiesis. It is suggested that CD34 is a signaling molecule involved in maintenance of a phenotypically plastic state in undifferentiated cells. CD34 is found in most endothelia, expressed on the luminal surface and membrane processes interdigitating between endothelial cells, but is absent from large veins and arteries. CD34 is furthermore expressed in fibroblast-like dendritic cells in, e.g., portal tracts of the liver, Peyer's patches, and in healing wounds. In smooth muscle cells, a variable CD34 staining is found. In tumor tissues, CD34 is detected in myeloid blasts in myelodysplastic syndrome and acute myeloid leukemia in most cases as well as lymphoblasts in most cases of B-acute lymphoblastic leukemia. Mature B- and T-cell lymphomas and leukemias are CD34 negative. The majority of vascular tumors, including hemangiosarcoma and Kaposi sarcoma are CD34positive. In a panel, CD34 staining is useful for the classification of myeloid and lymphoid neoplasms as well as spindle cell neoplasms (particularly identification of gastrointestinal stromal tumor and hemangiosarcoma).

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface
Gene Name
Positive Control
Jurkat or KG-1 cells. Human tonsil or angiosarcoma.
Immunogen
Synthetic peptide corresponding to CD34 residues within aa285-385 of CD34 was used as an immunogen
Alternate Names
Hematopoietic progenitor cell antigen CD34, Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Database Links

Entrez Gene ID
947
SwissProt

Additional Information

Clone
HPCA1/4393R
Chromosome Location
1q32.2
Mol. Weight of Antigen
41kDa

Functions

  • Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Key References

  • Felshow DM et al. Blood 97:3768-3775 (2001).
  • Sato T et al. Blood 94:2548-2554 (1999).

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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