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CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody [ICO-115]

Additional information

Catalog Number: 947-MSM1-CF647-100T
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
947-MSM1-CF647-100T
Purified Ab Conjugated to CF647
0.5ml at 100ug/ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD34 Antibody in Flow Cytometry (FC)

CD34 Antibody in Flow Cytometry (FC)

Flow Cytometry Analysis of KG-1 cells using CD34 Monoclonal Antibody (ICO-115) (red) and isotype control (green).

CD34 Antibody in SDS-PAGE

CD34 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified CD34 Monoclonal Antibody (ICO-115). Confirmation of Integrity and Purity of Antibody.

  • ELISA
  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Clone
ICO-115
Isotype
IgG1
Cellular Localization
Cell surface, Membrane
Gene Name
CD34
Positive Control
KG-1 cells. Human tonsil or angiosarcoma.
Immunogen
Blast cells of a chronic myeloid leukemia patient
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Key References
  • Frolova, E., Baryshnikov A.Y., Frenkel M. et al., Monoclonal antibody ICO-115 to human CD34 antigen. Herald of the Cancer Research Center of RAMS. Suppl, p. 10-12, 1994.
  • Kishimoto et al., Leucocyte typing Gariand Publishing. 1997.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
947
SwissProt
P28906
Chromosome Location
1q32.2
Mol. Weight of Antigen
90-110kDa
Alternate Names
Hematopoietic progenitor cell antigen CD34, Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Summary

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.

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