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Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody [HPCA1/2598R]

In Stock
Catalog Number Formulation Size Price
947-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
947-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
947-RBM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).

Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).

Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).

Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody - Image 4

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).

Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody - Image 5

Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).

Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody - Image 6

SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Integrity and Purity of Antibody.

Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody - Image 7

Flow Cytometric Analysis of human Jurkat cells using CD34 CD34 Recombinant Rabbit MAb (HPCA1/2598R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

CD34 Antibody in SDS-PAGE

CD34 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunohistochemistry
Species Reactivity
Human, Rat
Research Areas
Hypoxia, Immunology, Endothelial Cell Marker, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Membrane
Gene Name
CD34
Positive Control
Jurkat, KG-1 cells. Tonsil or Angiosarcoma.
Immunogen
Recombinant full-length human HPCA1 protein
Alternate Names
Hematopoietic progenitor cell antigen CD34, Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Database Links

Entrez Gene ID
947
SwissProt
P28906

Additional Information

Clone
HPCA1/2598R
Chromosome Location
1q32.2
Mol. Weight of Antigen
90-110kDa

Related Antibodies

View All CD34 Antibodies

Functions

  • Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Key References

  • Felshow DM et al. Blood 97:3768-3775 (2001).
  • Sato T et al. Blood 94:2548-2554 (1999).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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