2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)

Recombinant Rabbit Monoclonal Antibody [Clone S100A4/2750R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

img

Immunofluorescence staining of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

img

Western Blot Analysis of human HeLa cell lysate using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

img

Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

img

SDS-PAGE Analysis Purified S100A4Recombinant Rabbit Monoclonal Antibody (S100A4/2750R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

S100A4; S100 calcium-binding protein A4; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein

Positive Control

HeLa, A549 or A375 cells. Placenta.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic and Nuclear

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

10-12kDa

Specificity & Comments

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ismail NI, Kaur G, Hashim H, Hassan MS. S100A4 overexpression proves to be independent marker for breast cancer progression. Cancer Cell Int. 2008 Sep 5;8:12.

Bioinformatics

  •  Entrez Gene ID: 6275
  •  Gene Symbol: S100A4
  •  SwissProt: P26447
  •  Chromosome Location: 1q21
  •  Unigene: 654444
NBT02750