Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

Immunofluorescence staining of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Western Blot Analysis of human HeLa cell lysate using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

SDS-PAGE Analysis Purified S100A4Recombinant Rabbit Monoclonal Antibody (S100A4/2750R). Confirmation of Purity and Integrity of Antibody.

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) [S100A4/2750R]

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Summary

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Human, Mouse
Isotype
IgG
Cellular Localization
Cytoplasm, Nucleus, Secreted
Gene Name
S100A4
Mol. Weight of Antigen
10-12kDa
Immunogen
Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary)

Database Links

Entrez Gene ID
6275
Unigene
654444
SwissProt
P26447

Additional Information

Clone
S100A4/2750R
Human Gene Symbol
S100A4
Chromosome Location
1q21

Known Applications & Suggested Dilutions

  • Western Blot (1-2ug/ml)
  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Ismail NI, Kaur G, Hashim H, Hassan MS. S100A4 overexpression proves to be independent marker for breast cancer progression. Cancer Cell Int. 2008 Sep 5;8:12.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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