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S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody [S100A4/6797]

Additional information

Catalog Number: 6275-MSM7
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6275-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
6275-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6275-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
S100A4 Antibody in Western Blot (WB)

S100A4 Antibody in Western Blot (WB)

Western Blot Analysis of HeLa lysate using S100A4 Mouse Monoclonal Antibody (S100A4/6797).

S100A4 Antibody in Immunohistochemistry (IHC (P))

S100A4 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human spleen stained with S100A4 Mouse Monoclonal Antibody (S100A4/6797). Inset: PBS instead of primary antibody; secondary only negative control.

S100A4 Antibody in SDS-PAGE

S100A4 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified S100A4 Mouse Monoclonal Antibody (PAX7/13020). Confirmation of Purity and Integrity of Antibody.

S100A4 Antibody in Western Blot (WB)

S100A4 Antibody in Western Blot (WB)

Western Blot Analysis of Human Lung, A549 and HeLa lysates using S100A4 Mouse Monoclonal Antibody (S100A4/6797).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
S100A4/6797
Isotype
IgG2c
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
S100A4
Positive Control
HeLa cells. A549, Lung.
Immunogen
Recombinant fragment (around aa 1-101) of human S100A4 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:30713770, PubMed:26654597).
Key References
  • Ismail NI,Kaur G,Hashim H,Hassan MS. S100A4 overexpression proves to be independent marker for breast cancer progression. Cancer Cell Int.2008 Sep 5;8:12.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6275
SwissProt
P26447
Chromosome Location
1q21
Mol. Weight of Antigen
10-12kDa
Alternate Names
Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4, S100A4; S100 calcium-binding protein A4; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein

Summary

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, α6β4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

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