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S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody [S100A4/1482]

In Stock
Catalog Number Formulation Size Price
6275-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
6275-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
6275-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Mouse Monoclonal Antibody (S100A4/1482).

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 2

Western Blot of HeLa, A549 and A375 cell lysate using S100A4 Mouse Monoclonal Antibody (S100A4/1482).

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 3

SDS-PAGE Analysis Purified S100A4 Mouse Monoclonal Antibody (S100A4/1482). Confirmation of Integrity and Purity of Antibody.

S100A4 Antibody in Western blot (WB)

S100A4 Antibody in Western blot (WB)

Western Blot Analysis of HeLa cell lysate using S100A4 Mouse Monoclonal Antibody (S100A4/1482).

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 5

Flow Cytometric Analysis of T98G cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 6

Flow Cytometric Analysis of A549 cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 7

Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse
Research Areas
Dendritic Cell Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Nucleus, Secreted
Gene Name
S100A4
Positive Control
A549 or A375 cells. Placenta., HeLa
Immunogen
Recombinant fragment of human S100A4 protein (aa1-200) (exact sequence is proprietary)
Alternate Names
Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4, S100A4; S100 calcium-binding protein A4; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein

Database Links

Entrez Gene ID
6275
SwissProt
P26447

Additional Information

Clone
S100A4/1482
Chromosome Location
1q21
Mol. Weight of Antigen
10-12kDa

Related Antibodies

View All S100A4 Antibodies

Functions

  • Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:30713770, PubMed:26654597).

Key References

  • Ismail NI, Kaur G, Hashim H, Hassan MS. S100A4 overexpression proves to be independent marker for breast cancer progression. Cancer Cell Int. 2008 Sep 5;8:12.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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