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Home >> Antibodies >> ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target)

ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target)

Mouse Monoclonal Antibody [Clone ICOSL/3111]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with ICOS-L Mouse Monoclonal Antibody (ICOSL/3111).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ICOS-L Mouse Monoclonal Antibody (ICOSL/3111).

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Flow Cytometric Analysis of U937 cells using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence staining of U937 cells using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot

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SDS-PAGE Analysis Purified ICOS-L Mouse Monoclonal Antibody (ICOSL/3111). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111).

Product Details

Synonyms

B7 homolog 2 (B7H2); B7 like protein Gl50; B7 related protein 1 (B7RP1); CD275; CD275 antigen; GL50; ICOS LG; ICOS ligand (ICOSL); Inducible T cell co stimulator ligand; LICOS; Transmembrane protein B7 H2 ICOS ligand

Positive Control

U937, HL60, HuT78 and HUVEC lysates (WB). Human tonsil tissue (IHC).

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149) (exact sequence is proprietary)

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

This MAb recognizes a protein of 36kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yoshinaga, S.K., et al. 1999. T cell co-stimulation through B7RP-1 and ICOS. Nature 402: 827-832.
  2. Cao, Y., et al. 2018. Expression of B7-H2 on CD8+ T cells in colorectal cancer microenvironment and its clinical significance. Int Immunopharmacol 56: 128-13.

Bioinformatics

  •  Entrez Gene ID: 23308
  •  Gene Symbol: ICOSLG
  •  SwissProt: O75144
  •  Chromosome Location: 21q22.3
  •  Unigene: 14155
NBT03111