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CD68 (Macrophage Marker) Antibody [C68/684]

In Stock
Best Seller
Catalog Number Formulation Size Price
968-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
968-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
968-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml.
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Mouse Monoclonal Antibody (C68/684).

Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Mouse Monoclonal Antibody (C68/684).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Mouse Monoclonal Antibody (C68/684).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Mouse Monoclonal Antibody (C68/684).

SDS-PAGE Analysis Purified CD68 Mouse Monoclonal Antibody (C68/684).

SDS-PAGE Analysis Purified CD68 Mouse Monoclonal Antibody (C68/684).

Western Blot Analysis of human Spleen tissue lysate using CD68 Mouse Monoclonal Antibody (C68/684).

Western Blot Analysis of human Spleen tissue lysate using CD68 Mouse Monoclonal Antibody (C68/684).

Immunofluorescence staining of U87MG cells using CD68 Mouse Monoclonal Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot

Immunofluorescence staining of U87MG cells using CD68 Mouse Monoclonal Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot

Flow Cytometric Analysis of U87MG cells using CD163 Mouse Monoclonal Antibody (M130/3296) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of U87MG cells using CD163 Mouse Monoclonal Antibody (M130/3296) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocyticneoplasia. Lymphomas are negative or show few granules.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Monkey, Mouse, Rat
Research Areas
Immunology, Hematopoietic Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Endosome membrane, Lysosome membrane
Gene Name
CD68
Positive Control
lymph node or spleen., Tonsil, U87MG cells
Immunogen
Recombinant human CD68 protein
Alternate Names
Macrosialin, Gp110, GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1

Database Links

Entrez Gene ID
968
SwissProt
P34810

Additional Information

Clone
C68/684
Chromosome Location
17p13.1
Mol. Weight of Antigen
110kDa

Related Antibodies

View All CD68 Antibodies

Functions

  • Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Key References

  • Pulford KA et. al. Journal of Clinical Pathology, 1989, 42(4):414-21.
  • Warnke RA et. al. Am J of Pathol, 1989, 135:1089-95.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA &azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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