Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target) Antibody [ICOSL/3111]

In Stock
Catalog Number Formulation Size Price
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
Immunohistochemistry (IHC)
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes


This MAb recognizes a protein of 36kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

Product Properties & Targets

Antibody Type
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
Positive Control
HL60, HuT78 and HUVEC lysates (WB). Human tonsil tissue (IHC)., U937
Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149) (exact sequence is proprietary)
Alternate Names
ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, B7 homolog 2 (B7H2); B7 like protein Gl50; B7 related protein 1 (B7RP1); CD275; CD275 antigen; GL50; ICOS LG; ICOS ligand (ICOSL); Inducible T cell co stimulator ligand; LICOS; Transmembrane protein B7 H2 ICOS ligand

Database Links

Entrez Gene ID

Additional Information

Chromosome Location
Mol. Weight of Antigen


  • Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity).

Key References

  • Yoshinaga, S.K., et al. 1999. T cell co-stimulation through B7RP-1 and ICOS. Nature 402: 827-832.
  • Cao, Y., et al. 2018. Expression of B7-H2 on CD8+ T cells in colorectal cancer microenvironment and its clinical significance. Int Immunopharmacol 56: 128-13.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


There are no reviews yet.

Be the first to review “ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target) Antibody”

Your email address will not be published. Required fields are marked *


We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.