2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Mouse Monoclonal Antibody [Clone KRT7/903]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903)

img

Purified Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903). Confirmation of Integrity and Purity of Antibody.

img

Flow Cytometric Analysis of trypsinized methanol-fixed HeLa cells using Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

img

Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Product Details

Synonyms

CK7; K2C7; K7; Keratin 55k Type II Cytoskeletal; Keratin 7; Keratin Simple Epithelial Type 1 k7; Keratin Type II Cytoskeletal 7; Keratin, 55K Type II Cytoskeletal; Krt2-7; KRT7; Sarcolectin; SCL; Type ii Mesothelial Keratin k7; Type-II Keratin Kb7

Positive Control

A431, HeLa cells, Breast cancer.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT7 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Does not react with Mouse, Rat, Ferret or Rabbit.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

54kDa

Specificity & Comments

Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fuchs, E. 1995. Keratins and the skin. Annu. Rev. Cell Dev. Biol. 11:123-153.

Bioinformatics

  •  Entrez Gene ID: 3855
  •  Gene Symbol: KRT7
  •  SwissProt: P08729
  •  Chromosome Location: 12q13.13
  •  Unigene: 411501
NBT00903