Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30)
Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30)
Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).
SDS-PAGE Analysis Purified Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30). Confirmation of Integrity and Purity of Antibody
Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescent staining of methanol-fixed HeLa cells. KRT7 Mouse Monoclonal Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 7 Recombinant Mouse Monoclonal Antibody (OV-TL12/30). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of lung tissue lysates of different species using Cytokeratin-7 Mouse Monoclonal Antibody (OV-TL12/30).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
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