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Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [K72.7]

In Stock
Catalog Number Formulation Size Price
3855-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
3855-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
3855-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 3

Flow Cytometric Analysis of PFA-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (K72.7) followed by Goat anti-Mouse IgG-CF488 (blue); Isotype Control (red).

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 4

Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling KRT7 with KRT7 Mouse Monoclonal Antibody (K72.7) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 5

Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT7
Positive Control
cervix or breast (IHC)., HeLa cells (FC/IF). HeLa cell lysate (WB). Carcinoma of ovary, Lung
Immunogen
Semi-Purified cytokeratin preparation.
Alternate Names
Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Database Links

Entrez Gene ID
3855
SwissProt
P08729

Additional Information

Clone
K72.7
Chromosome Location
12q13.13
Mol. Weight of Antigen
55kDa

Related Antibodies

View All KRT7 Antibodies

Functions

  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Key References

  • Ramaekers F., et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 1990;136:641-55.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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