Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [SPM270]

In Stock
Catalog Number Formulation Size Price
3855-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
3855-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
3855-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (SPM270).

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 2

Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Monoclonal Antibody (SPM270).

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 3

Immunofluorescence Analysis of HeLa cells labeling Cytokeratin 7 with KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 4

Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Cytokeratin 7 Antibody in SDS-PAGE

Cytokeratin 7 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified KRT7 Mouse Monoclonal Antibody (SPM270).Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT7
Positive Control
cervix or breast., HeLa cells. Carcinoma of ovary, Lung
Immunogen
OTN 11, ovarian carcinoma cell line
Alternate Names
Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Database Links

Entrez Gene ID
3855
SwissProt
P08729

Additional Information

Clone
SPM270
Chromosome Location
12q13.13
Mol. Weight of Antigen
55kDa

Related Antibodies

View All KRT7 Antibodies

Functions

  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Key References

  • Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 1990;136:641-55

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales