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Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [KRT7/4387R]

These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.

The MAbMan recommends these antibodies for repeatable and reliable results.

Additional information

Catalog Number: 3855-RBM14
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
3855-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3855-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3855-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Formalin-fixed, paraffin-embeddedhuman bladdercarcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 4

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KRT7/4387R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Cytokeratin 7 Antibody in SDS-PAGE

Cytokeratin 7 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified KRT7 Recombinant Rabbit Monoclonal Antibody(KRT7/4387R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
KRT7/4387R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT7
Positive Control
Carcinoma of ovary, Cervix, Lung, or breast.
Immunogen
Recombinant fragment (around aa1-100) of human KRT7 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Reactivity:

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3
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Gene:

Host:

Reactivity:

, , ,

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Other Information

Functions
  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Key References
  • Moll R, et al. Histochem Cell Biol. 2008 Jun;129(6):705-33.
  • Moll R, et al. Cell. 1982 Nov;31(1):11-24.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3855
SwissProt
P08729
Chromosome Location
12q13.13
Mol. Weight of Antigen
55kDa
Alternate Names
Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Summary

Cytokeratin 7 in humans is a protein encoded by the KRT7 gene. It is always as a keratin pair with Cytokeratin 19. Cytokeratin 7 is expressed on most ductal and glandular epithelium including lung, breast, bladder and female genital tract, but not in most gastrointestinal epithelium, prostate, hepatocyte and squamous epithelium. In tumor tissues, its expression is absent in colon cancer, prostate cancer and squamous carcinomas. Cytokeratin 7 is often used in concert with Cytokeratin 20 and CDX-2 to aid in distinguishing ovarian, pulmonary and breast carcinomas (CK7+) from colon carcinomas (CK7-).

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