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Home >> Antibodies >> Actin, Muscle Specific (Muscle Cell Marker)

Actin, Muscle Specific (Muscle Cell Marker)

Mouse Monoclonal Antibody [Clone HHF35]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).

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Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).

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SDS-PAGE Analysis Purified Muscle Specific Actin Mouse Monoclonal Antibody (HHF35). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of Skeletal muscle tissue lysate using Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).

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Western Blot Analysis of Endometrium muscle tissue lysate using Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).

Product Details

Synonyms

ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1

Positive Control

Muscle or sarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

SDS extract of human myocardium.

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Rabbit. Cat. Dog. Mouse. Rat. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Multiple

Specificity & Comments

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tsukada T. et. al. Am J Pathol 1987, 126:51.
  2. Tsukada T. et. al. Am J Pathol 1987, 127:389.

Bioinformatics

  •  Entrez Gene ID: 58, 59, 70
  •  Gene Symbol: ACTA1 (Skeletal); ACTA2 (Smooth); ACTG2 (Smooth)
  •  SwissProt: P62736, P68032, P68133
  •  Chromosome Location: 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
  •  Unigene: 118127, 1288, 500483
NBT00445