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Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody [1A4 + ACTA2/791]

Additional information

Catalog Number: 59-MSM3
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
59-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
59-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
59-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody

Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded Rat Stomach stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - Image 4

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Smooth Muscle Actin MAb (1A4 + ACTA2/791).

Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - Image 5

Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with Smooth Muscle Actin MAb (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).

Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - Image 6

SDS-PAGE Analysis Purified Smooth Muscle Actin MAb (1A4 + ACTA2/791). Confirmation of Integrity and Purity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Rat
Clone
1A4 + ACTA2/791
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm, Cytoskeleton
Gene Name
ACTA2
Positive Control
Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
Immunogen
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH (1A4); Recombinant full-length human ACTA2 protein (ACTA2/791)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Key References
  • Gown, A.M., et al. 1985. A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes. J. Cell Biol. 100: 807-813.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
59
SwissProt
P62736
Chromosome Location
10q23.31
Mol. Weight of Antigen
42kDa
Alternate Names
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Summary

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

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