Actin, Muscle Specific (Muscle Cell Marker) [HHF35]

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Summary

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Gene Name
ACTA2
Mol. Weight of Antigen
Multiple
Immunogen
SDS extract of human myocardium.

Database Links

Entrez Gene ID

Additional Information

Clone
HHF35
Human Gene Symbol
ACTA1 (Skeletal); ACTA2 (Smooth); ACTG2 (Smooth)
Chromosome Location
1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Tsukada T. et. al. Am J Pathol 1987, 126:51.
  • Tsukada T. et. al. Am J Pathol 1987, 127:389.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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