| Catalog Number: | MSM1-445 |
|---|---|
| Reactivity: | |
| Gene: | |
| Host: |
Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).
Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).
SDS-PAGE Analysis Purified Muscle Specific Actin Mouse Monoclonal Antibody (HHF35). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Skeletal muscle tissue lysate using Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).
Western Blot Analysis of Endometrium muscle tissue lysate using Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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