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Actin, Muscle Specific (Muscle Cell Marker) Antibody [MSA/953]

In Stock
Catalog Number Formulation Size Price
MSM2-953-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM2-953-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM2-953-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Western blot analysis of hSKM cell lysate using Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Western blot analysis of hSKM cell lysate using Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Lung stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Lung stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Stomach stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Stomach stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Skeletal Muscle stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Formalin-fixed, paraffin-embedded Rat Skeletal Muscle stained with Muscle Specific Actin Mouse Monoclonal Antibody (MSA/953).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human, Rabbit, Rat
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytoskeleton
Gene Name
ACTA2
Positive Control
Muscle or sarcoma.
Immunogen
Synthetic peptide common among human muscle actins
Alternate Names
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1

Database Links

Entrez Gene ID
58, 59 & 70
SwissProt
P62736, P68032 & P68133

Additional Information

Clone
MSA/953
Chromosome Location
1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Mol. Weight of Antigen
Multiple

Related Antibodies

View All ACTA2 Antibodies

Functions

  • Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Key References

  • Barkalow, K. and Hartwig, J.H. 1995. Curr. Biol. 5: 1000-1002.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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