Formalin-fixed, paraffin-embedded human Prostate stained with p27 Mouse Monoclonal Antibody (SPM348).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling p27 with p27 Mouse Monoclonal Antibody (SPM348) followed by goat anti-mouse IgG-CF488. Membrane is stained with Phalloidin-CF640.

Printable Datasheet

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) [SPM348]

Name: p27Kip1 (Mitotic Inhibitor/Suppressor Protein)
SKU: 1027-MSM1X
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.

Product Properties & Targets

Host

Mouse

Applications

Immunofluorescence, Immunohistochemistry

Species Reactivity

Human, Monkey, Mouse, Rat

Research Areas

Cancer, Cardiovascular, Hypoxia

Isotype

IgG1

Cellular Localization

Cytoplasm, Endosome, Nucleus

Light Chain

Kappa

Gene Name

CDKN1B

Positive Control

Breast or Colon Ca., MCF7 cells. Tonsil, MDA-MB-231, SK-BR-3, T47D, ZR75

Immunogen

Purified GST-p27 fusion protein of Mouse origin

Alternate Names

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Database Links

Entrez Gene ID

1027

Unigene

238990

SwissProt

P46527

Additional Information

Clone

SPM348

Human Gene Symbol

CDKN1B

Chromosome Location

12p13.1

Mol. Weight of Antigen

25-26kDa

Functions

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Known Applications & Suggested Dilutions

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Fredersdorf S et. al. Proc Natl Acad Sci 1997;94:6380

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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