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p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody [KIP1/769]

Additional information

Catalog Number: 1027-MSM2-CF488
Host:

Reactivity:

, , ,

Gene:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
1027-MSM2-CF488-100T
Purified Ab conjugated to CF488
0.5ml at 100ug/ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
p27 Antibody in Flow Cytometry (FC)

p27 Antibody in Flow Cytometry (FC)

Flow Cytometry of human p27 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled p27 Monoclonal Antibody (KIP1/769).

p27 Antibody in SDS-PAGE

p27 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified p27 Mouse Monoclonal Antibody (KIP1/769). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human, Monkey, Mouse, Rat
Clone
KIP1/769
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
CDKN1B
Positive Control
Human tonsil, breast or colon carcinoma. MCF7, MDA-MB-231 or SK-BR-3 cells.
Immunogen
Recombinant human KIP1 protein
Storage & Stability
Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Key References
  • Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, et al. High level expression of p27Kip1and cyclin D1 in some human breast cancer cells: Inverse correlation between the expression of p27Kip1 and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci 1997;94:6380-5.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1027
SwissProt
P46527
Chromosome Location
12p13.1
Mol. Weight of Antigen
25-26kDa
Alternate Names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Summary

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

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