Contact Us

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody [DCS-72.F6 + KIP1/769]

Additional information

Catalog Number: 1027-MSM4
Reactivity:

, , ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
1027-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1027-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1027-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody

Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody - Image 2

SDS-PAGE Analysis of Purified p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Monkey, Mouse, Rat
Clone
DCS-72.F6 + KIP1/769
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm, Endosome, Nucleus
Gene Name
CDKN1B
Positive Control
Breast, Cervical or Colon Carcinoma., HeLa or MCF7 cells. Tonsil, MDA-MB-231, SK-BR-3, T47D, ZR75
Immunogen
Mouse recombinant p27 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

SDS-PAGE Analysis of Purified P-Cadherin Mouse Monoclonal Antibody (12H6). Confirmation of Integrity and Purity of Antibody.
0
  • Flow
  • IF
  • WB

P-Cadherin (CDH3) Antibody

Additional information

Catalog Number: 1001-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded Mouse Heart stained with N-Cadherin Mouse Monoclonal Antibody (CDH2/1573).
1
  • IHC

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM3
Reactivity:

,

Gene:

Host:

SDS-PAGE Analysis of Purified N-Cadherin Mouse Monoclonal Antibody (13A9). Confirmation of Integrity and Purity of Antibody.
1
  • Flow
  • IF
  • WB

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM1
Reactivity:

,

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4443). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM3
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded Human Renal Cell Carcinoma Stained with KSP-Cadherin Rabbit Polyclonal Antibody.
0
  • IHC

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBP3
Reactivity:

, ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (SPM594).
2
  • IHC
  • WB

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM1X
Reactivity:

, , , ,

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
3
  • Flow
  • IF

HDAC6 Antibody

Additional information

Catalog Number: 10013-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/3874R).
3
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-RBM5
Gene:

Host:

Reactivity:

, , ,

VIEW ALL ANTIBODIES

Other Information

Functions
  • Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Key References
  • Fredersdorf S et. al. Proc Natl Acad Sci 1997;94:6380-5.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1027
SwissProt
P46527
Chromosome Location
12p13.1
Mol. Weight of Antigen
25-26kDa
Alternate Names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Summary

Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us