Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody [KIP1/1355R]

In Stock
Catalog Number Formulation Size Price
1027-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
1027-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
1027-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody

SDS-PAGE Analysis of Purified p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R). Confirmation of Purity and Integrity of Antibody.

Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human bladder. Strong nuclear staining using KIP1/1355R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

p27 Antibody in Western Blot (WB)

p27 Antibody in Western Blot (WB)

Western blot analysis of A431 lysate using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).

p27 Antibody in Western Blot (WB)

p27 Antibody in Western Blot (WB)

Western Blot Analysis of NIH/3T3 cell lysate using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).

p27 Antibody in Western Blot (WB)

p27 Antibody in Western Blot (WB)

Western Blot analysis of Human Spleen and Human Sk. Muscle tissue lysates using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).

p27 Antibody in Western Blot (WB)

p27 Antibody in Western Blot (WB)

Western blot analysis of Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle, Hamster Skeletal Muscle, Guinea pig Skeletal Muscle, and Rabbit Skeletal Muscle tissue lysates using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- � � induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat
Research Areas
Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Infectious Disease, Lung Cancer, Nuclear Marker, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Endosome, Nucleus
Gene Name
CDKN1B
Positive Control
Breast, Cervical or Colon Carcinoma., NIH/3T3, HeLa or MCF7, A431 cells. Human tonsil, Human Spleen, Skeletal Muscle
Immunogen
Recombinant full-length human CDKN1B protein
Alternate Names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Database Links

Entrez Gene ID
1027
SwissProt
P46527

Additional Information

Clone
KIP1/1355R
Chromosome Location
12p13.1
Mol. Weight of Antigen
25-26kDa

Related Antibodies

View All CDKN1B Antibodies

Functions

  • Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Key References

  • Fredersdorf S et al. Proc Natl Acad Sci 1997;94:6380-5.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales