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p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody [SX53G8]

In Stock
Best Seller
Catalog Number Formulation Size Price
1027-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1027-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1027-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
p27 Antibody in Immunohistochemistry (IHC (P))

p27 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Mouse Monoclonal Antibody (SX53G8).

p27 Antibody in Immunohistochemistry (IHC (P))

p27 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Mouse Monoclonal Antibody (SX53G8).

p27 Antibody in Flow Cytometry (FC)

p27 Antibody in Flow Cytometry (FC)

Flow Cytometry of human p27 on HeLa cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Mouse Monoclonal Antibody (SX53G8).

p27 Antibody in Immunofluorescence (IF)

p27 Antibody in Immunofluorescence (IF)

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling p27 with p27 Mouse Monoclonal Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640

p27 Antibody in Flow Cytometry (FC)

p27 Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using p27 Mouse Monoclonal Antibody (SX53G8) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

p27 Antibody in SDS-PAGE

p27 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified p27 Mouse Monoclonal Antibody (SX53G8). Confirmation of purity and integrity.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Monkey, Mouse, Rat
Research Areas
Cancer, Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Infectious Disease, Lung Cancer, Nuclear Marker, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 /
Cellular Localization
Cytoplasm, Endosome, Nucleus
Gene Name
CDKN1B
Positive Control
ZR75
Immunogen
Purified GST-p27 fusion protein of human origin
Alternate Names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Database Links

Entrez Gene ID
1027
SwissProt
P46527

Additional Information

Clone
SX53G8
Chromosome Location
12p13.1
Mol. Weight of Antigen
25-26kDa

Related Antibodies

View All CDKN1B Antibodies

Functions

  • Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Key References

  • Fredersdorf S et al. Proc Natl Acad Sci 1997;94:6380-5.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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