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GM-CSF (Granulocyte/Macrophage – Colony Stimulating Factor) Antibody [BVD2-21C11]

In Stock
Catalog Number Formulation Size Price
1437-RTM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1437-RTM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1437-RTM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified GM-CSF Rat Monoclonal Antibody (BVD2-21C11). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified GM-CSF Rat Monoclonal Antibody (BVD2-21C11). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Granulocyte/macrophage – Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Product Properties & Targets

Antibody Type
Rat Monoclonals
Host
Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cynomolgus Monkey, Human, Rhesus Monkey
Research Areas
Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Hematopoietic Stem Cells, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Secreted
Gene Name
CSF2
Positive Control
Lymph node or tonsil.
Immunogen
Recombinant human GM-CSF protein
Alternate Names
Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, Molgramostin, Sargramostim, Burst Promoting Activity; Eosinophil Colony Stimulating Factor; Molgramostin; Pluripoietin Alpha; Sargramostim

Database Links

Entrez Gene ID
1437
SwissProt
P04141

Additional Information

Clone
BVD2-21C11
Chromosome Location
5q31.1
Mol. Weight of Antigen
22kDa

Related Antibodies

View All CSF2 Antibodies

Functions

  • Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Key References

  • Abrams J, et al. 1992. Immunol. Rev. 127:5.
  • Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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