GM-CSF (Granulocyte/Macrophage – Colony Stimulating Factor) [CSF2/3403]

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Summary

Granulocyte/macrophage – Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Product Properties & Targets

Host
Applications
Species Reactivity
Isotype
Cellular Localization
Secreted
Light Chain
Gene Name
Positive Control
Human spleen or tonsil.
Immunogen
Recombinant human GM-CSF protein
Alternate Names
Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, Molgramostin, Sargramostim, Burst Promoting Activity; Eosinophil Colony Stimulating Factor; Molgramostin; Pluripoietin Alpha; Sargramostim

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
CSF2/3403
Human Gene Symbol
CSF2
Chromosome Location
5q31.1
Mol. Weight of Antigen
22kDa

Functions

  • Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Abrams J, et al. 1992. Immunol. Rev. 127:5.
  • Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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