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Home >> Antibodies >> Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)

Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)

Mouse Monoclonal Antibody [Clone TOP2A/1362]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).

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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).

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Western Blot Analysis of human HeLa cell lysate using Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).

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Confocal Immunofluorescence image of HeLa cells using Topo II alpha, Monoclonal Antibody (TOP2A/1362). Green (CF488) and Phalloidin (Red) is used to label the nuclei.

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SDS-PAGE Analysis Purified Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Topoisomerase II alpha Mouse Monoclonal Antibody (TOP2A/1362).

Product Details

Synonyms

ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A

Positive Control

HeLa cells. Tonsil.

Known Applications & Suggested Dilutions

Western Blot, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human Topoisomerase II alpha (around aa1352-1493) (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

170kDa

Specificity & Comments

It recognizes a 170kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Chung, T.D., et al. 1989. Proc. Natl. Acad. Sci USA 86: 9431-9435.

Bioinformatics

  •  Entrez Gene ID: 7153
  •  Gene Symbol: TOP2A
  •  SwissProt: P11388
  •  Chromosome Location: 17q21.2
  •  Unigene: 156346
NBT01362