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Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody [TOP2A/7148R]

Additional information

Catalog Number: 7153-RBM8
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7153-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7153-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7153-RBM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Formalin-fixed, paraffin-embedded human lymph node stained with Topo IIa Recombinant Rabbit Monoclonal Antibody (TOP2A/7148R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

TOP2A Antibody in SDS-PAGE

TOP2A Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified DNA topoisomerase 2-alpha Recombinant Rabbit Monoclonal Antibody (TOP2A/7148R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
TOP2A/7148R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus.
Gene Name
TOP2A
Positive Control
Human tonsil or esophagus.
Immunogen
Synthetic peptide corresponding to Topo II α residues within aa1431-1531 of Topo II α
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
Key References
  • Lynch BJ, et al. Hum. Pathol. 1997 Oct;28(10):1180-8
  • Gupta D, et al. Appl. Immunohistochem. Mol. Morphol. 2001 Sep;9(3):215-21.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7153
SwissProt
P11388
Chromosome Location
17q21.2
Mol. Weight of Antigen
170kDa
Alternate Names
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; Topoisomerase DNA II alpha 170kDa; TP2A

Summary

The topoisomerase II-α isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-α is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-α is the predominant mechanism of resistance to several chemotherapeutic agents.

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