|Catalog No||Formulation||Size||Price (USD)|
|7153-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|7153-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|7153-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|Human Entrez Gene ID||7153|
|Human Gene Symbol||TOP2A|
|Human Chromosome Location||17q21.2|
|Synonyms||ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A|
|Immunogen||Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG2b, kappa|
|Mol. Weight of Antigen||170kDa|
|Species Reactivity||Human and Mouse. Others not known.|
It recognizes a 170kDa protein, which is identified as topoisomerase II. shows no cross-reaction with Topoisomerase II or I. Topo II plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II is also implicated in drug resistance of tumor cells.
Flow Cytometry (0.5-1ug/million cells)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
HeLa cells or Tonsil
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.