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Home >> Antibodies >> S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)

S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)

Recombinant Mouse Monoclonal Antibody [Clone rS100A4/1481]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).

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Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).

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Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence staining of A549 cells using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

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SDS-PAGE Analysis Purified S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of Panc-1 cell lysate using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).

Product Details

Synonyms

S100 calcium-binding protein A4; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein

Positive Control

HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)

Cellular Localization

Cytoplasmic and nuclear

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

10-12kDa

Specificity & Comments

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ismail NI, Kaur G, Hashim H, Hassan MS. S100A4 overexpression proves to be independent marker for breast cancer progression. Cancer Cell Int. 2008 Sep 5;8:12.

Bioinformatics

  •  Entrez Gene ID: 6275
  •  Gene Symbol: S100A4
  •  SwissProt: P26447
  •  Chromosome Location: 1q21
  •  Unigene: 654444
NBT03186