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Home >> Antibodies >> Phosphotyrosine (P-Tyr)

Phosphotyrosine (P-Tyr)

Mouse Monoclonal Antibody [Clone PY793]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified Phosphotyrosine Monoclonal Antibody (PY793). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Phosphotyrosine Monoclonal Antibody (PY793).

Product Details

Synonyms

P-Tyr

Positive Control

MCF-7, MDA-231, T47-D cells. Breast carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)Optimal dilution for a specific application should be determined.

Immunogen

Phosphotyrosine conjugated to BSA

Cellular Localization

Depends upon the location of phosphorylated target

Species Reactivity

All species

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

Depends upon the phosphorylated target

Specificity & Comments

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hunter, T., et al. 1985. Protein-tyrosine kinases. Annu. Rev. Biochem. 54: 897-930.

Bioinformatics

NBT00793