Phosphotyrosine (P-Tyr) [PY793]

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Summary

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
Depends upon the phosphorylated target
Immunogen
Phosphotyrosine conjugated to BSA

Database Links

Entrez Gene ID
Unigene
SwissProt
N/A

Additional Information

Clone
PY793

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)Optimal dilution for a specific application should be determined.

Key References

  • Hunter, T., et al. 1985. Protein-tyrosine kinases. Annu. Rev. Biochem. 54: 897-930.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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