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Home >> Antibodies >> Ep-CAM / CD326 (Epithelial Marker)

Ep-CAM / CD326 (Epithelial Marker)

Biotinylated Mouse Monoclonal Antibody [Clone VU-1D9]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

499.00

Formulation

Purified Ab conjugated to Biotin

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to Biotin

Price (USD)

499.00
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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Biotin-conjugated Ep-CAM Mouse Monoclonal Antibody (VU-1D9).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Positive Control

HT29 cells or breast tumor

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °
C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Small cell lung carcinoma cells

Cellular Localization

Cell surface

Species Reactivity

Human. Does not react with rat and ferret.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

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Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Key References

  1. Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
  2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.

Bioinformatics

  •  Entrez Gene ID: 4072
  •  Gene Symbol: TACSTD1
  •  SwissProt: P16422
  •  Chromosome Location: 2p21
  •  Unigene: 542050