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Recombinant EpCAM / CD326 (Epithelial Marker) Antibody [EGP40/8130R]

In Stock
Catalog Number Formulation Size Price
4072-RBM36-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
4072-RBM36-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
4072-RBM36-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant EpCAM / CD326 (Epithelial Marker) Antibody

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using EGP40/8130R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant EpCAM / CD326 (Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/8130R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant EpCAM / CD326 (Epithelial Marker) Antibody - Image 3

IHC analysis of formalin-fixed, paraffin-embedded human kidney. Negative tissue control using EGP40/8130R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface
Gene Name
EPCAM
Positive Control
MCF-7 or HT29 cells. Human breast tumor.
Immunogen
Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)
Alternate Names
Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Database Links

Entrez Gene ID
4072
SwissProt
P16422

Additional Information

Clone
EGP40/8130R
Chromosome Location
2q21
Mol. Weight of Antigen
40-43kDa

Related Antibodies

View All EPCAM Antibodies

Functions

  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Key References

  • Bjork, P., et al. 1993. J. Biol. Chem. 268: 24232-24241.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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