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EpCAM / CD326 (Epithelial Marker) Antibody [VU-1D9]

In Stock
Catalog Number Formulation Size Price
4072-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4072-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4072-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
EpCAM Antibody in Immunofluorescence (IF)

EpCAM Antibody in Immunofluorescence (IF)

Immunofluorescence Analysis of MCF-7 cells. EpCAM Mouse Monoclonal Antibody (VU-1D9) labeled with CF488 (green); NucSpot is used to label the nuclei (red).

EpCAM / CD326 (Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Biotin-conjugated Ep-CAM Mouse Monoclonal Antibody (VU-1D9).

EpCAM / CD326 (Epithelial Marker) Antibody - Image 3
EpCAM Antibody in Flow Cytometry (FC)

EpCAM Antibody in Flow Cytometry (FC)

Flow cytometric analysis of MCF-7 cells. Unstained cells (gray); CF555-labeled Ep-CAM-stained cells (VU-1D9) (orange).

EpCAM Antibody in Flow Cytometry (FC)

EpCAM Antibody in Flow Cytometry (FC)

Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray); CF555-labeled EpCAM (VU-1D9) (orange).

EpCAM Antibody in SDS-PAGE Analysis

EpCAM Antibody in SDS-PAGE Analysis

SDS-PAGE Analysis of Purified EpCAM Mouse Monoclonal Antibody (VU-1D9). Confirmation of Purity and Integrity of Antibody.

Schematic representation of epitope recognized by EpCAM antibody

Schematic representation of epitope recognized by EpCAM antibody

Schematic representation of EpCAM and epitope recognized by EpCAM Mouse Monoclonal Antibody (VU-1D9).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Stem Cell Differentiation
Isotype / Light Chain
IgG1 /
Cellular Localization
Cell junction, Cell surface, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
HT29 cells or breast tumor, HT29 cells. Breast or Colon Carcinoma.
Immunogen
Small cell lung carcinoma cells
Alternate Names
Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Database Links

Entrez Gene ID
4072
SwissProt
P16422

Additional Information

Clone
VU-1D9
Chromosome Location
2p21
Mol. Weight of Antigen
40-43kDa

Related Antibodies

View All EPCAM Antibodies

Functions

  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Key References

  • Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
  • Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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