Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Formalin-fixed, paraffin-embedded canine (dog) bladder stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Formalin-fixed, paraffin-embedded feline (cat) small intestine stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

SDS-PAGE Analysis Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of MCF-7 cell lysate using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Western blot analysis of human lung tissue lysate using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

EpCAM / CD326 (Epithelial Marker) [EGP40/2041R]

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Summary

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

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Product Properties & Targets

Host
Rabbit
Species Reactivity
Cat, Dog, Human
Isotype
IgG
Cellular Localization
Cell junction, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Mol. Weight of Antigen
40-43kDa
Immunogen
Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

Database Links

Entrez Gene ID
4072
Unigene
542050
SwissProt
P16422

Additional Information

Clone
EGP40/2041R
Human Gene Symbol
TACSTD1
Chromosome Location
2q21

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Bjork, P., et al. 1993. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also availableWITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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