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Home >> Antibodies >> Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Biotinylated Mouse Monoclonal Antibody [Clone OV-TL12/30]

Catalog #


0.5 ml at 100ug/ml

Price (USD)



Purified Ab conjugated to Biotin

Catalog No


0.5 ml at 100ug/ml


Purified Ab conjugated to Biotin

Price (USD)


Formalin-fixed, paraffin-embeddedhuman prostatecarcinoma stained with Biotin-conjugated CK7 Mouse Monoclonal Antibody (OV-TL12/30).


Formalin-fixed, paraffin-embeddedhuman prsotatecarcinoma stained with Biotin-conjugated CK7 Mouse Monoclonal Antibody (OV-TL12/30).


SDS-PAGE Analysis Purified CK7 Mouse Monoclonal Antibody (OV-TL12/30) (unconjugated). Confirmation of Integrity and Purity of Antibody

Product Details


CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Positive Control

HeLa cells. Carcinoma of ovary, lung, cervix, or breast.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (2-4ug/ml)
Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °
C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.


OTN 11, ovarian carcinoma cell line

Cellular Localization


Species Reactivity


Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen


Specificity & Comments

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Related Products

Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Key References

  1. Ramaekers F, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 1990;136:641-55


  •  Entrez Gene ID: 3855
  •  Gene Symbol: KRT7
  •  SwissProt: P08729
  •  Chromosome Location: 12q13.13
  •  Unigene: 411501