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Home >> Antibodies >> Cyclin E (G1/S-Phase Cyclin)

Cyclin E (G1/S-Phase Cyclin)

Mouse Monoclonal Antibody [Clone CCNE1/2460]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E Mouse Monoclonal Antibody (CCNE1/2460).

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E Mouse Monoclonal Antibody (CCNE1/2460).

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Western Blot Analysis of human Jurkat cell lysate using Cyclin E Mouse Monoclonal Antibody (CCNE1/2460).

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SDS-PAGE Analysis Purified Cyclin E Mouse Monoclonal Antibody (CCNE1/2460). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Cyclin E Mouse Monoclonal Antibody (CCNE1/2460).

Product Details

Synonyms

CCNE1; Cyclin E1; G1/S-specific cyclin-E1

Positive Control

Jurkat, MCF-7 or HeLa cells. Human breast or colon carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

53kDa

Specificity & Comments

Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Camacho F I, Garcia J F, S nchez-Verde L, et al. American Journal of Pathology. 158 (4): 1363 1369 (2001).

Bioinformatics

  •  Entrez Gene ID: 898
  •  Gene Symbol: CCNE1
  •  SwissProt: IDP24864
  •  Chromosome Location: 19q12
  •  Unigene: 244723