Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Formalin-fixed, paraffin-embedded human endometrium stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Western Blot Analysis of HCT-116 cell lysate using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

SDS-PAGE Analysis Purified Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

Cyclin E (G1/S-Phase Cyclin) [CCNE1/4935R]

Name: Cyclin E (G1/S-Phase Cyclin)
SKU: 898-RBM5
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Nucleus

Gene Name

CCNE1

Mol. Weight of Antigen

53kDa.

Immunogen

Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Database Links

Entrez Gene ID

898

Unigene

244723

SwissProt

IDP24864

Additional Information

Clone

CCNE1/4935R

Human Gene Symbol

CCNE1

Chromosome Location

19q12

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.